Study of the minority variants: Detection of virus subpopulations with resistance mutations that are not detectable using conventional sequencing methods and its implications on the choice of a first line antiretroviral treatment, as well as on the choice of regimen upon successive failures.

 Resistance in the integrase gene: Detection of primary resistance, resistance mutations and basal polymorphisms using conventional and ultrasensitive methods.

 Genotype determination of viral tropism: Advances in the inference of viral tropism by the study of the V3 region of the HIV envelope. Application of ultrasensitive sequencing methods.

 Primary and subtype resistance: Coordination at a national level of epidemiological monitoring of primary resistance and HIV subtypes.